Preferred isoschizomer ?
ableasby at hgmp.mrc.ac.uk
ableasby at hgmp.mrc.ac.uk
Mon Apr 14 18:17:54 UTC 2003
1) If your colleague had explicitly said -enzymes psti
on the command line (or equivalent GUI) then it would
be found. The output would be overly verbose if all
isoschizomers are reported so as a compromise it reports
only one.
2) If you take the emboss files from the REBASE (NEB) distro
then, after renaming and putting them in data/REBASE, it will
probably report PstI (haven't tried it). I arranged with NEB
that they would provide only the 'common' REs in their files.
I believe this is what some other packages do. Using REBASEEXTRACT
on the withrefm file gives all the REs.
3) You can equate any reported RE to another by adding an entry
into embossre.equ e.g.
BspMAI PstI
HTH
Alan
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