which restriction sites are used ?
gbottu at ben.vub.ac.be
Mon Oct 7 10:03:10 UTC 2002
from : BEN
I am using EMBOSS version 2.4.1. I noticed the following : ApeKI is a
restriction enzyme without commercial provider. If I do "redata ApeKI" I get :
Recognition site is GCWGC leaving sticky ends
Cut positions 5':0 3':0 [5':0 3':0]
Organism: Aeropyrum pernix K1
Source: ATCC 700893
TseI AceI Taq52I TseBI
Kawarabayasi, Y. et al., (1999) DNA Res., vol. 6, pp. 83-101.
Xu, S.-Y., Opitz, L., Unpublished observations.
So, it is there and has a cut site that should match GCAGC.
Yet, when I run remap or restrict with parameters -nocommercial -nolimit on a
sequence containg GCAGC the restriction site is not found. Asking explicitly to
look for enzyme ApeKI does not help, the enzyme is not even mentioned as "enzyme
that does not cut".
Is that normal ?
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