Reading frames (for Gary)

James Bonfield jkb at
Fri May 3 13:34:19 UTC 2002

Hi all,

I've spoken to Rodger about how he reports open reading frames and he said
that his -2 starts at the same base as +2 - ie the reading frames are worked
out before complementing. (Infact the way his algorithms work avoids the need
to complement anyway as he just looks up using a reversed table - I think).

Gap4 does much the same thing too in it's own translation windows.


James Bonfield (jkb at   Fax: (+44) 01223 213556
Medical Research Council - Laboratory of Molecular Biology,
Hills Road, Cambridge, CB2 2QH, England.
Also see Staden Package WWW site at

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