[Bioperl-l] Conversion of Phred 33 -> Phred 64 quality
Fields, Christopher J
cjfields at illinois.edu
Mon Jan 26 15:18:06 UTC 2015
Though, see my more detailed reply. There are performance issues that need to be addressed.
> On Jan 26, 2015, at 8:35 AM, Peter Cock <p.j.a.cock at googlemail.com> wrote:
> Yes, BioPerl's SeqIO understands the two legacy formats "fastq-solexa"
> and "fastq-illumina" plus the original and now universal standard
> See also http://dx.doi.org/10.1093/nar/gkp1137
> On Mon, Jan 26, 2015 at 2:23 PM, Mark A. Jensen <maj at fortinbras.us> wrote:
>> Hi folks,
>> I know I could RTFM, but maybe someone knows off the top of their head: I
>> understand that Illumina at one time made a switch in the constant added to
>> quality scores to generate the FASTQ that comes off their instruments. This
>> leads to a certain incomparability of data before and after that switch.
>> This is about all I know of the issue; does anyone here have experience with
>> this? Are there any BP modules that do this translation?
>> much appreciated-
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