[Bioperl-l] Conversion of Phred 33 -> Phred 64 quality
p.j.a.cock at googlemail.com
Mon Jan 26 14:35:52 UTC 2015
Yes, BioPerl's SeqIO understands the two legacy formats "fastq-solexa"
and "fastq-illumina" plus the original and now universal standard
See also http://dx.doi.org/10.1093/nar/gkp1137
On Mon, Jan 26, 2015 at 2:23 PM, Mark A. Jensen <maj at fortinbras.us> wrote:
> Hi folks,
> I know I could RTFM, but maybe someone knows off the top of their head: I
> understand that Illumina at one time made a switch in the constant added to
> quality scores to generate the FASTQ that comes off their instruments. This
> leads to a certain incomparability of data before and after that switch.
> This is about all I know of the issue; does anyone here have experience with
> this? Are there any BP modules that do this translation?
> much appreciated-
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