[Bioperl-l] Bio::DB::SeqFeature sequences with no identifier?
Mark Wilkinson
markw at illuminae.com
Wed May 14 09:22:23 UTC 2014
Hi all BioPerlers!
I'm confused by something. In the scenario below I have a Fasta file
and a GFF file:
=========
File: a.fas
>SEQ1
AAAATTTTCCCCGGGG
=========
File: b.gff
SEQ1 hit1 match_part 1 5 . . . .
SEQ1 hit2 match_part 6 10 . . . .
=========
I load them into a seqfeature DB:
bp_seqfeature_load.pl -d dbi:mysql:seqdb -c -u root -p pass a.fas b.gff
I then explore the data as follows:
use Bio::DB::SeqFeature::Store;
my $db = Bio::DB::SeqFeature::Store->new(
-adaptor => 'DBI::mysql',
-dsn => 'dbi:mysql:seqdb',
-user => 'root',
-password => 'pass');
my $iterator = $db->get_seq_stream();
while (my $feature = $iterator->next_seq){
print $feature->seq->seq;
# THE SEQUENCE IS PRINTED
print " comes from sequence named ";
print $feature->seq->id;
# THE METHOD ABOVE RETURNS UNDEF
}
my $seq = $db->segment('SEQ1');
# $seq is undef, NOTHING IS RETURNED!?!?
============
This is all very confusing. It seems that the feature knows what
sequence it is attached to, because it gives me the correct string of
letters, but it doesn't know what the name of that sequence is... and in
fact, calling the sequence by name returns undef.
Is this a bug, or is there a reason for this "disconnect" between a
sequence and its name?
Help appreciated!
Mark
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