It almost works, Just a little correction if this can help someone else: $rc = Bio::Seq->new( -seq => $hsp->hit_string )->revcom->seq; Thanks! -- View this message in context: http://bioperl.996286.n3.nabble.com/Problem-with-revcom-tp17581p17583.html Sent from the Bioperl-L mailing list archive at Nabble.com.