[Bioperl-l] Little question about Bio::SeqIO::fastq

Florent Angly florent.angly at gmail.com
Mon May 21 23:14:11 UTC 2012


Hi Juan,

Have a look at the beginners howto and the SeqIO howto here:
http://www.bioperl.org/wiki/HOWTOs

Regardless of what sequence format you use (fastq, fasta, etc), SeqIO 
can be used in the same fashion to read, edit and write sequence ID, 
description and sequence string.
Best,

Florent


On 21/05/12 23:32, Juan Jovel wrote:
> Hello All!
> When using 'Bio::SeqIO::fastq' how can I access each of the lines of a fastq entries?
> I need to split multiplexed libraries from MiSeq runs (which outputs in fastq format), and therefore I want to access the 'id' line of each sequence (@) to be able to split the file according to Illumina indices. How to do that?
> thanks a lot in advance.
> Juan  		 	   		
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> Bioperl-l at lists.open-bio.org
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