[Bioperl-l] Command line error in BLAST+

Frank Schwach fs5 at sanger.ac.uk
Fri Sep 16 08:36:37 UTC 2011

Hi Manju,

Are you on Ubuntu? I think I've seen problems with this bzip library on
Ubuntu before. It's not a problem with BLAST in any case. 
Should be possible to install the missing files through your package
manager. I'm sure Google will know what to do :)
Not sure what went wrong with your blast installation. What happens if
you run blastall directly (without the legacy_blast.pl script)? In any
case, it might be better to ask the NCBI people for help with the BLAST
installation as this is not a BioPerl problem.



On Fri, 2011-09-16 at 01:12 -0400, Manju Rawat wrote:
> Hi,
> I have installed the latest version of blast and download the database
> correctly Using this tutorial
> http://www.ncbi.nlm.nih.gov/staff/tao/URLAPI/unix_setup.html
> But when i running blastn-help command in terminal it showing me error that
> blastn: error while loading shared libraries: libbz2.so.1: cannot open
> shared object file: No such file or directory.
> and when i am running the blastall command then it showing that
> *legacy_blast.pl blastall -p blastn -i amino.fa -d nr -o blast.out*
> Can't exec "/usr/bin/blastn": No such file or directory at /usr/bin/
> legacy_blast.pl line 85.
> Program failed, try executing the command manually.
> While i have set the path of environment variable
> PATH=$PATH:/home/abc/ncbi-blast-2.2.25+/bin
> I have checked everything but not able tp fine the error..
> Pl help me.
> Thanks
> Manju
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