[Bioperl-l] Doubt - Regarding Stand Alone Blast

[sukeerthi teja Rallapalli]

Dear Sir,

I am trying to create a stand alone database in Mac using Blast.

I have installed Blast. I have pasted my 2 files (input file and the large
database file) also in the same folder as in Blast.

So far these are the commands that i have used are these

*"**formatdb -i sequences.fasta -p T -o T *
*
*
*
*

*blastall -p blastp -d homologene_result.fasta -i sequences.fasta -o stdout
-a 1 -e 10*

*
*

*
*

*OR*

*
*

 *makeblastdb -in** **sequences.fasta** **-dbtype **prot*

*
*

*
*

*blastn -query** test **-db **homologene_result.fasta** -out** stdout **-outfmt
6**"*


I think these are the two versions of blast .. But i am using the latest
version of Blast on my system.


I am always getting an error report, this is what it is.


*"**sukeerthi-tejas-macbook-pro:bin sukeerthiteja$ blastall -p blastp -d
homologene_result.fasta -i sequences.fasta -o stdout -a 1 -e 10*

*[NULL_Caption] WARNING: Unable to open homologene_result.fasta.pin*

*BLASTP 2.2.16 [Mar-25-2007]*

*
*

*
*

*Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, *

*Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), *

*"Gapped BLAST and PSI-BLAST: a new generation of protein database search*

*programs",  Nucleic Acids Res. 25:3389-3402.*

*
*

*Query= gi|293651546|ref|NP_001170840.1| nuclear factor NF-kappa-B p100*

*subunit isoform a [Mus musculus]*

*         (899 letters)*

*
*

*[NULL_Caption] WARNING: gi|293651546|ref|NP_001170840.1|: Unable to open
homologene_result.fasta.pin**"*

*
*

Please help me understand.


Thanking you

Teja