[Bioperl-l] problem in using protparam.pm module

[Shachi Gahoi]

Dear All,

i am using protparam.pm module. but when i am running this script it is
printing one error message

"Can't call method "throw" without a package or object reference at
/usr/share/perl5/Bio/Root/Root.pm line 368, <GEN1> line 1."

Kindly help me to solve this problem.


Script is here----
###################################################################################
#!/usr/bin/perl

use warnings;
use Bio::SeqIO;
use Bio::Tools::Protparam;


$seqfile='test1.fasta';

$seqio = Bio::SeqIO->new(-file => "$seqfile", -format => 'Fasta');


    while(  $seq = $seqio->next_seq() )
{


        my $pp = Bio::Tools::Protparam->new(-seq=>$seq->seq);

        print
        "ID : ", $seq->display_id,"\n",
        "Amino acid number : ",$pp->amino_acid_number(),"\n",
        "Number of negative amino acids : ",$pp->num_neg(),"\n",
        "Number of positive amino acids : ",$pp->num_pos(),"\n",
        "Molecular weight : ",$pp->molecular_weight(),"\n",
        "Theoretical pI : ",$pp->theoretical_pI(),"\n",
        "Total number of atoms : ", $pp->total_atoms(),"\n",
        "Number of carbon atoms : ",$pp->num_carbon(),"\n",
        "Number of hydrogen atoms : ",$pp->num_hydrogen(),"\n",
        "Number of nitrogen atoms : ",$pp->num_nitro(),"\n",
        "Number of oxygen atoms : ",$pp->num_oxygen(),"\n",
        "Number of sulphur atoms : ",$pp->num_sulphur(),"\n",
        "Half life : ", $pp->half_life(),"\n",
        "Instability Index : ", $pp->instability_index(),"\n",
        "Stability class : ", $pp->stability(),"\n",
        "Aliphatic_index : ",$pp->aliphatic_index(),"\n",
        "Gravy : ", $pp->gravy(),"\n",
        "Composition of A : ", $pp->AA_comp('A'),"\n",
        "Composition of R : ", $pp->AA_comp('R'),"\n",
        "Composition of N : ", $pp->AA_comp('N'),"\n",
        "Composition of D : ", $pp->AA_comp('D'),"\n",
        "Composition of C : ", $pp->AA_comp('C'),"\n",
        "Composition of Q : ", $pp->AA_comp('Q'),"\n",
        "Composition of E : ", $pp->AA_comp('E'),"\n",
        "Composition of G : ", $pp->AA_comp('G'),"\n",
        "Composition of H : ", $pp->AA_comp('H'),"\n",
        "Composition of I : ", $pp->AA_comp('I'),"\n",
        "Composition of L : ", $pp->AA_comp('L'),"\n",
        "Composition of K : ", $pp->AA_comp('K'),"\n",
        "Composition of M : ", $pp->AA_comp('M'),"\n",
        "Composition of F : ", $pp->AA_comp('F'),"\n",
        "Composition of P : ", $pp->AA_comp('P'),"\n",
        "Composition of S : ", $pp->AA_comp('S'),"\n",
        "Composition of T : ", $pp->AA_comp('T'),"\n",
        "Composition of W : ", $pp->AA_comp('W'),"\n",
        "Composition of Y : ", $pp->AA_comp('Y'),"\n",
        "Composition of V : ", $pp->AA_comp('V'),"\n",
        "Composition of B : ", $pp->AA_comp('B'),"\n",
        "Composition of Z : ", $pp->AA_comp('Z'),"\n",
        "Composition of X : ", $pp->AA_comp('X'),"\n";
}
###################################################################################




-- 
Regards,
Shachi