[Bioperl-l] Bioperl 1.6.1 problem

[Galbáts Borisz]

Dear Sir or Madam!

I'm new to Bioperl and got two probably very obvious problem (but I can't
find the solution). I installed bioperl 1.6.1 to Win XP (ActivePerl 5.12).
I hope I wrote to the appropriate e-mail address to discuss my problem.

I copy my code here and the error message:

#!/usr/bin/perl

use warnings;
use Bio::Perl;

$seq = get_sequence('swissprot', 'P43780');
write_sequence(">P43.fasta",'fasta',$seq);


-------------------------(run the script)----------------------

C:\Documents and Settings\Galbáts Borisz>perl biotest.perl

------------- EXCEPTION -------------
MSG: WebDBSeqI Error - check query sequences!

STACK Bio::DB::WebDBSeqI::get_seq_stream
C:/Perl/site/lib/Bio/DB/WebDBSeqI.pm:50
8
STACK Bio::DB::WebDBSeqI::get_Stream_by_acc
C:/Perl/site/lib/Bio/DB/WebDBSeqI.pm
:314
STACK Bio::DB::WebDBSeqI::get_Seq_by_acc
C:/Perl/site/lib/Bio/DB/WebDBSeqI.pm:18
6
STACK Bio::Perl::get_sequence C:/Perl/site/lib/Bio/Perl.pm:520
STACK toplevel biotest.perl:11
-------------------------------------

(if I use EMBL database, it works well)





My second problem is the following (script):

#!/usr/bin/perl
use warnings;
use Bio::Perl;

$blast_result=blast_sequence(CTTCCCTTTACCTGTGCACCACTCCCTAATAAATTCATCTCCATTGGGAAA);
write_blast(">bl.txt",$blast_result);

-------------------------(run the script)----------------------

...........................ck to sign
in"onclick="MyNCBI_auto_submit('http://www.ncbi.nlm.nih.gov/sites/myn
cbi/?back_url=http%3A//blast.ncbi.nlm.nih.gov/Blast.cgi%3FALIGNMENTS%3D50%26ALIG
NMENT%5FVIEW%3DPairwise%26CMD%3DPut%26COMPOSITION%5FBASED%5FSTATISTICS%3Doff%26D
ATABASE%3Dnr%26DESCRIPTIONS%3D100%26ERROR%3DMessage%2BID%252324%2BError%253A%2BF
ailed%2Bto%2Bread%2Bthe%2BBlast%2Bquery%253A%2BNucleotide%2BFASTA%2Bprovided%2Bf
or%2Bprotein%2Bsequence%26EXPECT%3D1e%2D10%26FILTER%3DL%26FORMAT%5FOBJECT%3DAlig
v/" title="National Library of
Me............................................. (quite long part)
dicine">NLM</a> |      <a href="http://www.nih.gov/" title="National
Institutes
of Health">NIH</a> |      <a href="http://www.hhs.gov/" title="US
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 feedback</a>         </p></div>           </div><!--/#wrap--><script
type="text
/javascript"
src="http://www.ncbi.nlm.nih.gov/coreweb/javascript/nsuggest/nsugge
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---------------------------------------------------
Submitted Blast for [blast-sequence-temp-id]



It seems it works but the output file (bl.txt) is always empty, whatever
is the input sequence. The problem is I have no idea what should I expect
when I run the script.



Thank you for you help in advance.

Your sincerely Borisz Galbáts (Hungary)