Set the E-value to a much lower number. Thomas (Tom) Keller, PhD kellert at ohsu.edu 503.494.2442 6588 R Jones Hall (BSc/CROET) www.ohsu.edu/xd/research/research-cores/dna-analysis/ On Jul 7, 2011, at 9:00 AM, <bioperl-l-request at lists.open-bio.org> wrote: > limit the number of blast output per query