[Bioperl-l] parsing entrezgene file (lost data)

[Smithies, Russell]

It is in there, just takes a bit of getting at.
Frequent use of Data::Dumper to work out where you are helps.



use warnings;
use strict;
use Bio::ASN1::EntrezGene;
use Data::Dumper;

my $parser = Bio::ASN1::EntrezGene->new('file' => "entrezgene.asn");
while(my $result = $parser->next_seq){
    $result = $result->[0] if(ref($result) eq 'ARRAY');
    foreach my $l (@{$result->{locus}}){
        foreach my $p (@{$l->{products}}){

          my $nuc_gi = $p->{seqs}->[0]->{whole}->[0]->{gi};
          my $nuc_acc = $p->{accession};

          my $prot_gi = $p->{products}->[0]->{seqs}->[0]->{whole}->[0]->{gi};
          my $prot_acc = $p->{products}->[0]->{accession};

          print "$nuc_gi, $nuc_acc\t$prot_gi, $prot_acc \n";
        }
    }
}



--Russell

> -----Original Message-----
> From: bioperl-l-bounces at lists.open-bio.org [mailto:bioperl-l-
> bounces at lists.open-bio.org] On Behalf Of Carnë Draug
> Sent: Monday, 4 July 2011 2:39 p.m.
> To: bioperl mailing list
> Subject: [Bioperl-l] parsing entrezgene file (lost data)
> 
> Hi
> 
> I've been trying to get some data from an ASN.1 entrezgene file.
> However, I can't seem to access some of the data on the file.  I've
> read the Feature-annotations page on the wiki (even fixed a bug in
> there) but still nothing. So I used Data::Dumper to look at the Seq and
> Annotation objects and couldn't see it in there at all although it's on
> the original file (attached).
> 
> The data I want from the sequence are the ids "NM_002105" and
> "NP_002096" which show up several times on the file. However, when I do
> this:
> 
> use Data::Dump;
> use Bio::SeqIO;
> my $file = $ARGV[0];
> my $seqio_object = Bio::SeqIO->new(-file => $file, -format =>
> 'entrezgene'); my $seq_object = $seqio_object->next_seq; print
> Dumper($seq_object);
> 
> I can't find 002105 or 002096 anywhere on the output.
> 
> Am I doing something wrong? How can I solve this?
> 
> Thanks in advance,
> Carnë Draug
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