[Bioperl-l] Output of a BLAST parse to text file
T.Hori
tiago.hori at gmail.com
Sun Apr 10 22:56:43 UTC 2011
Hi Guys,
I am really new to BioPerl, so this may be a stuoid question. Let's
say I use the example from the BioPerl.org:
use strict;
use Bio::SearchIO;
my $in = new Bio::SearchIO(-format => 'blast',
-file => 'report.bls');
while( my $result = $in->next_result ) {
## $result is a Bio::Search::Result::ResultI compliant object
while( my $hit = $result->next_hit ) {
## $hit is a Bio::Search::Hit::HitI compliant object
while( my $hsp = $hit->next_hsp ) {
## $hsp is a Bio::Search::HSP::HSPI compliant object
if( $hsp->length('total') > 50 ) {
if ( $hsp->percent_identity >= 75 ) {
print "Query=", $result->query_name,
" Hit=", $hit->name,
" Length=", $hsp->length('total'),
" Percent_id=", $hsp->percent_identity, "\n";
}
}
}
}
}
That gives me several hits as results on the standard output. I am
starting to learn BioPerl for what I think is a dauting task. I have a
colletion of 20K ESTs and I have to find the best Human hit for
everyone of those sequences. So I am starting by learning how to parse
BLAST results. I was wondering how I would go about having the output
of the parse go to tab-delimeted text file instead of the standard
output.
Any help would be greatly appreciated.
Thanks,
Tiago
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