[Bioperl-l] Bio::Tools::Run::Primer3 vs Web Primer 3

[Dave Messina]

Hi Veronica,

Without having checked to be sure, my guess is that either the version of Primer3 on the website is different from the one you're running locally or the default parameters on the website are different from the default parameters when running via B::T::R::Primer3.

If you run Primer3 on your machine outside of BioPerl — that is, just on the command line directly — do you get the same results as on the website?


Dave