[Bioperl-l] SeqIO::abi use

Cook, Malcolm MEC at stowers.org
Fri Apr 9 18:36:30 UTC 2010


I don't think so, but c.f. http://search.cpan.org/~vita/Bio-Trace-ABIF-1.05/lib/Bio/Trace/ABIF.pm#raw_data_for_channel%28%29 


Malcolm Cook
Stowers Institute for Medical Research -  Bioinformatics
Kansas City, Missouri  USA
 

-----Original Message-----
From: bioperl-l-bounces at lists.open-bio.org [mailto:bioperl-l-bounces at lists.open-bio.org] On Behalf Of Tom Keller
Sent: Thursday, April 08, 2010 5:30 PM
To: BioPerl-List
Subject: [Bioperl-l] SeqIO::abi use

Greetings,
Can the Bio::SeqIO::abi module be used to access the raw fluorescence data? Are there any examples available? I'm wondering how to calculate ratios of mixed bases prior to the smoothing and scaling done by the base caller.

thank you
Tom
kellert at ohsu.edu<mailto:kellert at ohsu.edu>
503-494-2442








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