[Bioperl-l] [rt.cpan.org #36216] Unable to parse score from BLAST output file
Chris Fields via RT
bug-bioperl at rt.cpan.org
Tue May 27 18:10:04 UTC 2008
Queue: bioperl
Ticket <URL: http://rt.cpan.org/Ticket/Display.html?id=36216 >
Please submit this using the BioPerl bugzilla:
http://bugzilla.open-bio.org/
Attach a full BLAST example to the bug report so we can track it.
On Tue May 27 13:52:36 2008, kajack wrote:
> Hi,
>
> I think there is a small bug in the Bio::SearchIO module. I am parsing
> the BLAST output file using this module. It works great except for one
> thing.
>
> I have included a part of the blast outputfile ( I have modified the
> lines to fit into this box). Most of the times the score of Sequences
> producing significant alignments is in the format of 6.149e+04. This
> module picks up only 6 and ignores other digits.
>
> Can you please look into this for me.
>
> Thank you in advance
> Kashi
>
> Attached: The sample Blast output file is here
>
> ====================================================================
> BLASTN 2.2.15 [Oct-15-2006]
>
> Query= Contig_1011 (31,018 letters)
>
> Database: scaffold_3.fsa
> 84 sequences; 3,615,155 total letters
>
> Searching..................................................done
>
>
>
> Score E
> Sequences producing significant alignments: (bits) Value
>
> Contig_1011 6.149e+04 0.0
> Contig_8873 2397 0.0
> Contig_1482 2042 0.0
> Contig_9461 1475 0.0
> Contig_1977 339 7e-92
>
> >Contig_1011
> Length = 31018
>
> Score = 6.149e+04 bits (31018), Expect = 0.0
> Identities = 31018/31018 (100%)
> Strand = Plus / Plus
>
>
> Query: 1
cttcaacaaacacgtatttctgaatgaaattgtttagagtttgttgaaggtcacgatcag 60
> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
> Sbjct: 1
cttcaacaaacacgtatttctgaatgaaattgtttagagtttgttgaaggtcacgatcag 60
>
>
> Query: 61
> gctcatagaccagcggtcctgaaagaggattgcctttaagtttgttggaaaaaacgatta 120
> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
> Sbjct: 61
> gctcatagaccagcggtcctgaaagaggattgcctttaagtttgttggaaaaaacgatta 120
>
>
> ====================================================================
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