[Bioperl-l] [rt.cpan.org #36216] Unable to parse score from BLAST output file
Kashi Revanna via RT
bug-bioperl at rt.cpan.org
Tue May 27 17:52:38 UTC 2008
Tue May 27 13:52:36 2008: Request 36216 was acted upon.
Transaction: Ticket created by kajack
Queue: bioperl
Subject: Unable to parse score from BLAST output file
Broken in: (no value)
Severity: (no value)
Owner: Nobody
Requestors: kashi.mail at gmail.com
Status: new
Ticket <URL: http://rt.cpan.org/Ticket/Display.html?id=36216 >
Hi,
I think there is a small bug in the Bio::SearchIO module. I am parsing
the BLAST output file using this module. It works great except for one
thing.
I have included a part of the blast outputfile ( I have modified the
lines to fit into this box). Most of the times the score of Sequences
producing significant alignments is in the format of 6.149e+04. This
module picks up only 6 and ignores other digits.
Can you please look into this for me.
Thank you in advance
Kashi
Attached: The sample Blast output file is here
====================================================================
BLASTN 2.2.15 [Oct-15-2006]
Query= Contig_1011 (31,018 letters)
Database: scaffold_3.fsa
84 sequences; 3,615,155 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Contig_1011 6.149e+04 0.0
Contig_8873 2397 0.0
Contig_1482 2042 0.0
Contig_9461 1475 0.0
Contig_1977 339 7e-92
>Contig_1011
Length = 31018
Score = 6.149e+04 bits (31018), Expect = 0.0
Identities = 31018/31018 (100%)
Strand = Plus / Plus
Query: 1 cttcaacaaacacgtatttctgaatgaaattgtttagagtttgttgaaggtcacgatcag 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1 cttcaacaaacacgtatttctgaatgaaattgtttagagtttgttgaaggtcacgatcag 60
Query: 61
gctcatagaccagcggtcctgaaagaggattgcctttaagtttgttggaaaaaacgatta 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 61
gctcatagaccagcggtcctgaaagaggattgcctttaagtttgttggaaaaaacgatta 120
====================================================================
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