[Bioperl-l] HELP: script cannot find primer3, which has been properly installed
lzhtom at hotmail.com
Mon Sep 25 03:46:28 UTC 2006
i installed primer3_1.0b and bioperl-run-1.4 following the instructions.
the source of primer3 executables is in
then i tried the script in the documentation for bio::tool::run::primer3
my $seqio = Bio::SeqIO->new(-file=>'input.1');
my $seq = $seqio->next_seq;
my $primer3 = Bio::Tools::Run::Primer3->new(-seq => $seq,
-outfile => "temp.out",
my $results = $primer3->run;
but when i ran it, there was an error message saying that primer3 couldn't
i'm pretty sure primer3 has been properly installed. 'cause when i went to
the source directory /home/zz/setup/primer3_1.0b/src/, i could run the
program there using:
why couldn't the perl script find primer3?
thanks a lot!
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