[Bioperl-l] Blast information
Brian Osborne
bosborne11 at verizon.net
Wed Oct 18 17:10:34 UTC 2006
Kevin,
Are you looking for hsp_length()? See the SearchIO HOWTO for a list of
methods:
http://www.bioperl.org/wiki/HOWTO:SearchIO
Brian O.
On 10/18/06 11:16 AM, "Kevin Brown" <Kevin.M.Brown at asu.edu> wrote:
> I just recently upgraded to 1.5.1 on WinXP to bring this version closer
> to live to parse some locally created blast files. I'm trying to find
> the method that returns the values that are underneath the Identities
> and Positives information as I'm trying to replicate the output of an
> old blast parser we have here written in RealBasic which is showing its
> age. Once I have it replicating the old output I then intend to add
> more features in terms of filtering returned hits (like not returning
> self->self hits or a->b so don't show b->a).
>
> Example:
> I'm looking for the methods that will return 117 from identities and 117
> from positives. I can't just use num_identical/percent_identity as that
> isn't 100% accurate.
>
>> BurkM_2016
> Length = 241
>
> Score = 43.2 bits (88), Expect = 7e-005
> Identities = 26/117 (22%), Positives = 51/117 (43%)
>
> Query: 298 QEEFFYAFEALVANKAQVIITSDTYPKEISGIDDRLISRFDSGLTVAIEPPELEMRVAIL
> 357
> Q F F + A+ ++ + + + L +R GL + P E + A+L
> Sbjct: 111 QIALFNLFNEVRAHPMTALVVAGPAAPLALDVREDLRTRLGWGLVFHLAPLTDEGKAAVL
> 170
>
> Query: 358 MRKAQSEGVSLSEDVAFFVAKHLRSNVRELEGALRKILAYSKFHGREITIELTKEAL 414
> A+ G++L++DV ++ H R ++ L L + +S R +T+ L + L
> Sbjct: 171 KHAAKERGIALADDVPSYLLTHFRRDMPSLMSLLDALDRFSLEQKRAVTLPLLRAML 227
>
> Thanks,
> Kevin
>
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