[Bioperl-l] extracting subsequences

[Jason Stajich]

Bio::DB::Fasta is the best way to do this for big sequences.

-jason
On Oct 25, 2005, at 4:48 PM, Amit Indap wrote:

> Hi,
>
> I have to extract subsequences from fasta files containing entire
> human chromosomes. For example I would like to extract bp
> 167506667..167523040. I know how to do this using the Bio::Seq and
> Bio::SeqIO APIs. The problem is it takes a long time to read in an
> entire fasta file containing a chromosome. Is there a way I can speed
> this up?
>
> The bp indices are taken from BLAT-ing my sequences to the genome. I
> could use megablast to find which contigs my sequences lie on, and
> then read in those files rather than the whole chromosome.
>
> Any suggestions would be helpful. Thanks.
>
> Amit
> --
> Amit Indap
> http://www.bscb.cornell.edu/Homepages/Amit_Indap/
>
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--
Jason Stajich
Duke University
http://www.duke.edu/~jes12