[Bioperl-l] cannot find path to blastall
Tim Erwin
taerwin at tpg.com.au
Wed Jun 22 03:14:52 EDT 2005
Hi Hafiz
You could try setting the executable with the code:
$factory->executable(’/some_dir/blastall’);
Otherwise you could use a symbolic link in /usr/local/bin
to point to your blastall exe.
i.e.
$> cd /usr/local/bin
$> ln -s /some_dir/blastall .
Regards,
Tim
On Wed, 2005-06-22 at 07:44 +0100, hafiz yusof wrote:
> I'm trying to set up a standalone blast and I'm
> getting an error message;
>
> MSG: cannot find path to blastall
>
> and i have running this code under Linux and i also
> haven't find blastall file in /usr/local/bin/, what
> should i do?
>
>
>
> code:
>
>
>
> #!/usr/bin/perl
>
> use strict;
> use Bio::SeqIO;
> use Bio::Tools::Run::StandAloneBlast;
>
> my $Seq_in = Bio::SeqIO->new (-file
> =>"/home/hafiz/bioperl/fasta", -format => 'fasta');
> my $query = $Seq_in->next_seq();
>
>
> my $factory =
> Bio::Tools::Run::StandAloneBlast->new('program' =>
> 'blastp',
>
> 'database' => 'ecoli.nt',
>
> _READMETHOD => "Blast"
> );
> my $blast_report = $factory->blastall($query);
> my $result = $blast_report->next_result;
>
> while( my $hit = $result->next_hit()) {
> print "\thit name: ", $hit->name(), "
> significance: ",
>
> $hit->significance(), "\n";}
>
>
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