[Bioperl-l] A error with Bio::Graphics::Pane, help me!!!!!

Lincoln Stein lstein at cshl.edu
Sat Jan 15 22:14:56 EST 2005


This happens when you try to create an image with height or width of 
zero.  Check to make sure that your sequence has positive length.

Lincoln

On Friday 14 January 2005 12:22 pm, Danielucg Sousa wrote:
> HI,
>
> I have done my code with image_and_map of
>
> Bio::Graphics::Pane Classe. So, out this message:
> > gd-png:  fatal libpng error: Image width or height
>
> is zero in IHDR
>
> > gd-png error: setjmp returns error condition
>
> My code until this erro is:
> #!/usr/bin/perl -wT
>
> use strict;
> use Bio::Graphics;
> use Bio::SeqIO;
> use Bio::SeqFeature::Generic;
> #use CGI ':standard';
> use CGI::Pretty;
>
> my $file = '/var/www/cgi-bin/AL391145.gb';
> my $io = Bio::SeqIO->new(-file=>$file);
> my $seq = $io->next_seq;
> #my $wholeseq =
> Bio::SeqFeature::Generic->new(-start=>1,-end=>$seq->length);
> my @features = $seq->all_SeqFeatures;
>  my $q = new CGI;
>
> # sort features by their primary tags
> my %sorted_features;
> for my $f (@features) {
>   my $tag = $f->primary_tag;
>   push @{$sorted_features{$tag}},$f;
> }
>
> print $q->header('text/html');
> print $q->start_html('A Vector Rendering ');
>
> my $panel =
> Bio::Graphics::Panel->new(-length=>$seq->length,
> -width       => 1000,				      -pad_left    => 10,
>      -pad_right   => 10,				      -key_color   =>
> 'white',				-key_spacing => 15,				      -key_style
> => 'bottom',			      -spacing     => -0.25,
> -box_subparts => 'true'				      );
> my ($url,$map,$mapname) = $panel->image_and_map(-root
> => '/home/bioinfo/cgi-bin',-url => '/tmpimages', );
>
> ----
> I don't know what I have to do. Please somebody help
> me.
>
> By,
> Daniel
>
>
>
>
>
>
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-- 
Lincoln D. Stein
Cold Spring Harbor Laboratory
1 Bungtown Road
Cold Spring Harbor, NY 11724

NOTE: Please copy Sandra Michelsen <michelse at cshl.edu> on
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