[Bioperl-l] Alignment Blast parser
Jason Stajich
jason at cgt.duhs.duke.edu
Thu Jan 22 14:14:14 EST 2004
You want to retain it meaning what?
Bio::SearchIO(-format => 'blast')
parses blast for you.
-jason
On Thu, 22 Jan 2004, Agrin, Nathan wrote:
> I need a way to retain the EXACT alignment found in a blast file.
> Ex-
>
>
> Query: 1 MAGRGK-GKTSGKKAVSRSAKAGLQFPVGRIARYLKKGKYAERIGAGAPVYLAAVLEYLT
> 59
> M+GRGK G + KA SRS++AGLQFPVGR+ R L+KG YAER+GAGAPVYLAAVLEYLT
> Sbjct: 1 MSGRGKQGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLT
> 60
>
>
> Query: 60 AEVLELAGNAARDNKKNRIVPRHIQLAIRNDEELGKLLGEVTIASGGVLPNIHAVLLP
> 117
> AE+LELAGNAARDNKK RI+PRH+QLAIRNDEEL KLLG VTIA GGVLPNI AVLLP
> Sbjct: 61 AEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGRVTIAQGGVLPNIQAVLLP
> 118
>
> I have tried everything to no avail. AlignIO almost works out, but
> instead of doing a typical blast alignment, the best it can do is a
> clustalw like alignment.
>
> Any help is much appreciated.
>
> -Nate
>
> Nathan Agrin
> Research Associate
> UMass Medical Center
> 55 Lake Ave. N.
> Worcester MA, 01655
> (508)-856-6018
> nathan.agrin at umassmed.edu
>
>
--
Jason Stajich
Duke University
jason at cgt.mc.duke.edu
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