[Bioperl-l] Bio::Graphics HOWTO :: about of the color of graph
Koichi Minamijima
bio at masrao.homelinux.com
Sat Oct 11 06:07:24 EDT 2003
Hi.
If it knows, please let me know.
I looked at the web page of "BioGraphics HOWTO."
It was used having edited "Example 4."
http://bioperl.org/HOWTOs/html/Graphics-HOWTO.html#parsing-
blast
[next unless $hit->significance < 1E-20;]
of the 38th line was only changed into
[next unless $hit->significance < 1;].
rscu.fas (ftp://ftp.ncbi.nlm.nih.gov/refseq/cumulative/rscu.fas.Z)
and "One arrangement file" were used, and blastall was done.
The picture was generated when "Exsample 4." was performed using the
result.
↓The picture of a result↓
http://masrao.com/img/render4.png (total)
or
http://masrao.com/img/render4.jpg (A part)
↓A part of result of BLAST↓
//-------------------------------------------start--------------------
-----------------------//
>gi|32563818|ref|NP_871912.1| (NM_182112) ROLler: helically
twisted,animals roll when moving ROL-8, COLlagenstructural gene (rol-8)
[Caenorhabdtis elegans]
Length = 265
Score = 70.1 bits (170), Expect = 2e-11
Identities = 37/95 (38%), Positives = 56/95 (58%), Gaps = 8/
95 (8%)
Query: 11
EVGRFRRNAFSAVALSTSCILAIVVLTPIAYQAIQRIHSNLLNDAHFCQARNRDLWTETM
70
E + RR AF AV +ST+ ++A VV P+ Y +Q S+L+ + +C+
AR+RD+W E
Sbjct: 8
EQRQMRRIAFVAVVVSTAAVIASVVTLPMLYNYVQSFQSHLMVETDYCKARSRDMWLEMT
67
Query: 71 KMARSRGRDEELVARTKRAVKGTWLFGQYIPDRSS 105
+ +G R KRA WLFGQ+IP+ S+
Sbjct: 68 ALQAGKGMGH----RMKRA----WLFGQWIPETSA 94
Score = 59.7 bits (143), Expect = 3e-08
Identities = 24/37 (64%), Positives = 25/37 (67%)
Query: 291 PGPYGKPGQPGAPGPDGSAGEKGGCDHCPPPRTPPGY 327
PG G+ G G GP G GEKG C HCPPPRTPPGY
Sbjct: 229 PGHEGRAGPNGPAGPPGDNGEKGDCGHCPPPRTPPGY 265
>gi|17533809|ref|NP_496362.1| (NM_063961) COLlagen
structural gene(29.2 kD) (col-82) [Caenorhabditis elegans]Length = 306
Score = 57.8 bits (138), Expect = 1e-07
Identities = 23/36 (63%), Positives = 24/36 (66%)
Query: 292 GPYGKPGQPGAPGPDGSAGEKGGCDHCPPPRTPPGY 327
G G+PG G GP G G GGCDHCPPPRT PGY
Sbjct: 271 GAAGQPGAAGEQGPAGEPGAGGGCDHCPPPRTAPGY 306
Score = 42.7 bits (99), Expect = 0.004
Identities = 20/76 (26%), Positives = 38/76 (50%)
Query: 10
LEVGRFRRNAFSAVALSTSCILAIVVLTPIAYQAIQRIHSNLLNDAHFCQARNRDLWTET
69
+E R+ AF + +ST L +V P+ Y +Q + S L + FC+ R L+ +
Sbjct: 12
VEAESLRKVAFFGICISTVATLTAIVAIPLLYNYMQHVQSTLQTEVDFCKHRTNGLFDQY
71
Query: 70 MKMARSRGRDEELVAR 85
+M + +G +L+ +
Sbjct: 72 ERMQQLKGVRGKLIVK 87
//---------------------------------------------------end--------------
-----------------------//
Please compare the portions of 292-327 of NM_182112 and NM_063961.
Graph is white, although NM_182112 are higher as for a score and e-
value is low.
It pats, although it is written to "http://bioperl.org/HOWTOs/html/
Graphics-HOWTO.html#addingscale" as "We specify the base background
color (-bgcolor => 'blue') and and the minimum and maximum scores to
scale to. (-min_score and-max_score)", and it is ?
Is it different in HSP?
It is a long text and is pardon.
Teach, if there are those who understand some.
It is it and English which is hard to read, and is pardon.
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