[Bioperl-l] parsing coded_by subfeature
Jack Chen
chenn at cshl.edu
Sun Jul 20 15:33:43 EDT 2003
Thanks Jason! I have looked into the script before but it does not
actually handle the join cases though.
I am also curious how to handle the cases where the 'coded_by' subfeature
contains the ">" and "<" signs. I am not really sure what they mean. And I
noticed that wherever these signs appear, the protein sequences retrieved
are different from the conceptual translation from the nucleotide
sequences. For example:
[nchen at whey blast_db_checked]$ ./test.pl "gi|8573628|gb|AAF77462.1|"
Protein obtained from GenBank:
MPQMAPISWLLLFIIFSITFILFCSINYYSYMPNSPKSNELKNINLNSMNWKW
CDS sequence is:
ATCCCACAAATAGCACCAATTAGATGATTATTACTATTTATTATTTTTTCTATTACATTTATTTTATTTTGTTCTATTAATTATTATTCTTATATGCCAAATTCACCTAAATCTAATGAATTAAAAAACATCAATTTAAATTCAATAAACTGAAAATGATAA
Conceptual translation is:
IPQIAPIR*LLLFIIFSITFILFCSINYYSYMPNSPKSNELKNINLNSIN*K**
Jack
++++++++++++++++++++++++++++++++++++++++++++
o-o Jack Chen, Stein Laboratory
o---o Cold Spring Harbor Laboratory
o----o #5 Williams, 1 Bungtown Road
O----O Cold Spring Harbor, NY, 11724
0--o Tel: 1 516 367 8394
O e-mail: chenn at cshl.org
o-o Website: http://www.wormbase.org
+++++++++++++++++++++++++++++++++++++++++++++
On Sun, 20 Jul 2003, Jason Stajich wrote:
> See the FAQ this question #5.4
> http://www.bioperl.org/Core/Latest/FAQ.html#Q5.4
>
> -jason
> On Sat, 19 Jul 2003, Jack Chen wrote:
>
> > Hi All,
> >
> > I'd like to retrieve nucleotide sequence for a give protein sequence. I
> > know that I could do it through coded_by subfeature, which can be rather
> > messy. Say, it could be one of the following formats
> >
> > #AF264924.1:1749..>2110
> > #AF264924.1:<254..1563
> > #join(AY260053.1:497..545,AY260053.1:610..3342,
> > AY260053.1:3409..3750,AY260053.1:3810..4511, AY260053.1:4569..4960)
> >
> > Is their a good and unified way to do it?
> >
> > Thanks
> >
> > Jack
> >
> >
> > _______________________________________________
> > Bioperl-l mailing list
> > Bioperl-l at portal.open-bio.org
> > http://portal.open-bio.org/mailman/listinfo/bioperl-l
> >
>
> --
> Jason Stajich
> Duke University
> jason at cgt.mc.duke.edu
>
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