[Bioperl-l] problems with parsing hmmpfam
Sofia
sofia at neuro.utah.edu
Fri Aug 1 13:59:46 EDT 2003
I am trying to parse a hmmpfam report. I am mostly sucessful until I have the following error:
Use of uninitialized value in array element at /usr/lib/perl5/site_perl/5.8.0/Bio/SearchIO/hmmer.pm line 488, <GEN1> line 56473.
------------- EXCEPTION -------------
MSG: Somehow the Model table order does not match the order in the domains (got [no, expected AFP)
STACK Bio::SearchIO::hmmer::next_result /usr/lib/perl5/site_perl/5.8.0/Bio/SearchIO/hmmer.pm:489
STACK toplevel ./parseHMM.pl:8
--------------------------------------
It seems to only occur when my scores for sequence family classification is "no hits above threshold" and my parsed for domains has a hit (though usually very poor score).
How can I filter out these records with SearchIO?
Thanks,
Sofia
Output which causes me to have this error
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Query sequence: 26SrRNA
Accession: [none]
Description: [none]
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
[no hits above thresholds]
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
AFP 1/1 268 279 .. 1 12 [] 0.3 89
Alignments of top-scoring domains:
AFP: domain 1 of 1, from 268 to 279: score 0.3, E = 89
*->tCTgStnCteAt<-*
tCT +t Ct+A
26SrRNA 268 TCTCGTACTGAG 279
//
Query sequence: 40Sribosomal.protS11
Accession: [none]
Description: [none]
Scores for sequence family classification (score includes all domains):
Model Description Score E-value N
-------- ----------- ----- ------- ---
[no hits above thresholds]
Parsed for domains:
Model Domain seq-f seq-t hmm-f hmm-t score E-value
-------- ------- ----- ----- ----- ----- ----- -------
AFP 1/1 127 138 .. 1 12 [] 1.7 53
Alignments of top-scoring domains:
AFP: domain 1 of 1, from 127 to 138: score 1.7, E = 53
*->tCTgStnCteAt<-*
+ Tg+tnC +
40Sribosom 127 AATGGTNCATTA 138
//
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
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