[Bioperl-l] e-value from RemoteBlast differs from hand made blast

[Carsten Daub]

I don't use any local database.
I blast (a) with the perl programm and (b) by pasting the same
sequence into my browser window at NCBI.

Cheers
Carsten

Martin Schenker schrieb:

> Might be a stupid question, but have you checked that you are using the same
> BLAST version and database in both instances?
>
> The BLAST version is given in the first line of the output like:
>
> TBLASTN 2.2.1 [Apr-13-2001]
>
> The database stats are given below the Query:
>
> Database: /scratch/db/my_strange_db
>            719,859 sequences; 375,894,912 total letters
>
> Best, Martin
>
> > -----Original Message-----
> > From: Carsten Daub [SMTP:daub@mpimp-golm.mpg.de]
> > Sent: Wednesday, May 22, 2002 11:57 AM
> > To:   Perl - Bioperl
> > Cc:   daub@mpimp-golm.mpg.de
> > Subject:      [Bioperl-l] e-value from RemoteBlast differs from hand made
> > blast
> >
> > Hi,
> >
> > I blasted with RemoteBlast and the score/e-values I obtain differ from
> > the ones
> > I get by a hand made blast at NCBI.
> > Is there a way to get the the same score/e-value by RemoteBlast as by
> > hand-made blast?
> >
> > Carsten
>
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Carsten Daub

MPI of Molecular Plant Physiology
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