[Biojava-l] Problem with reversecomplement

[Richard Holland]

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Could you provide the sequences you are working with, including the
sequence you are expecting and the actual sequence you are getting? It's
hard to tell what is wrong without seeing the actual sequences involved.

Also I'm not sure what you mean in your second question about the
sequence panel. What class are you working with? Is there a method you
have tried but doesn't work, or is it that you cannot find the method to
do what you want to do?

cheers,
Richard

abhinav wrote:
> hello all,
> I am using the biojava library to get the reversecomplement of a 
> sequence.The problem is I am not getting the required reverse complement.
> I am converting an ab1 file into a trace and from that I am generating 
> the sequence for the particular file.
> Then I am applying the subsequence() method to get the subsequence and 
> am applying the reversecomplement ()function on the particular 
> subsequence so that the two sequences that I am getting using the blast 
> shouls match up.But I am not getting the correct Reverse Complement.
> Is there anything wrong with this approach?
> Another thing is I am not able to set the ruler in the sequence panel to 
> go from max to min.i.e from say 550 to 0.How do I go about it?
> Thanks a lot in advance.
> _______________________________________________
> Biojava-l mailing list  -  Biojava-l at lists.open-bio.org
> http://lists.open-bio.org/mailman/listinfo/biojava-l
> 
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