[Biojava-l] Problem With Isoelectric point

[yasset perez riverol]

 Hi, 
 I have a problem when I execute the Isoelectrc
 Point calculation for this peptides:
    ALLIDLPV
    FDIYAGTPV
    SVLLMSAEI
    PTGDTTGYLANPEEV
 I all the case I receive this error :
    Error, the peptide probably contains only positive
or negative charges.
 
Well I try to calculate the isoelectric Point for
all the peptides with de Expasy Calculator and I
obtained:
       ALLIDLPV: 
          Theoretical pI: 3.80
          Molecular weight (average): 853.07 
       FDIYAGTPV: 
          Theoretical pI: 3.80
          Molecular weight (average): 982.10 
       SVLLMSAEI: 
          Theoretical pI: 4.00
          Molecular weight (average): 962.17 
       PTGDTTGYLANPEEV: 
          Theoretical pI: 3.57
          Molecular weight (average): 1563.64
 
 My code is this:
   OBioSeq = ProteinTools.createProtein(Sequence);
   IsoelectricPointCalc OBioIsoc = new
  IsoelectricPointCalc();
   double dIsoelec = 0.0;
   dIsoelec = OBioIsoc.getPI(OBioSeq,true,true);
   
 Thanks in advance
 Center of Molecular Biology.
 Havana University
 Cuba.


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