[Biojava-l] Re:topic about RestrictionEnzyme
yezhenqing at hotmail.com
Fri May 13 20:48:15 EDT 2005
maybe it's not suitable for me to answer this question. but i am glad to
my understanding here.(just my guess)
to be a biologist, it is right for the defination of overhangs in the
but in the case involved in a cut type where the enzyme cuts in two
relative to the recognition site, i think it's inconvenient to code
uniform according to biological rules.
you can try more enzymes in the code, maybe you will find the rules of
in the code implementation.
For example HgaI GACGC (5/10) indicates cleavage as follows:
5' GACGCNNNNN^ 3'
3' CTGCGNNNNNNNNNN^ 5'
it should be 5'-overhang according to biological rule, but in the code
3'-overhang. i think the defination of overhang in the code implementation
is related with
the strand direction.
anyway, it's not harmful to our biological tasks, and not confused for us
to solve problems
when we keep the difference in mind.
Ãâ·ÑÏÂÔØ MSN Explorer: http://explorer.msn.com/lccn/
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