[Biojava-l] Protein digest
Robert Stones
r.stones at csl.gov.uk
Thu Sep 11 06:24:00 EDT 2003
Hi
Does anyone have any example code to digest proteins and work out the
mass of the peptides generated?
biojava-l-request at portal.open-bio.org wrote:
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> Today's Topics:
>
> 1. RE: Best way to represent multiple annotation "tracks"
> (Ihab A.B. Awad)
> 2. Reformating multiple sequence alignments (Gabriel del Rio Guerra)
> 3. Parsing location from gbk files
> ( charles.girardot at libertysurf.fr )
> 4. RE: Reformating multiple sequence alignments (Schreiber, Mark)
> 5. RE: Parsing location from gbk files (Schreiber, Mark)
> 6. Re: Parsing location from gbk files (Matthew Pocock)
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Mon, 8 Sep 2003 16:53:09 -0700
> From: "Ihab A.B. Awad" <ihab at stanford.edu>
> Subject: RE: [Biojava-l] Best way to represent multiple annotation
> "tracks"
> To: "Schreiber, Mark" <mark.schreiber at agresearch.co.nz>,
> <biojava-l at biojava.org>
> Message-ID: <p05210608bb82c3fa2305@[172.21.30.101]>
> Content-Type: text/plain; charset="us-ascii" ; format="flowed"
>
> Hi again,
>
> [ Pls excuse the poor netiquette for posting a followup to my own message.... ]
>
> >So, let's say I have a bunch of ESTs, each of which has been mapped
> >to a location on the genome. To each EST, my data maps a floating
> >point number indicating the expression level.
> >
> >In this case, each Feature would represent one of the ESTs, right?
> >So what would be the Feature(s) representing the annotation track?
> >Would they be Features owned by the EST Feature (via the inheritance
> >of FeatureHolder by interface Feature)? Or -- ??
>
> Alternatively, would each EST be a SubSequence with Features attached
> to it, with each Feature representing one of the data "tracks"? If
> so, then how does one decide when something is a Feature or a
> SubSequence? I'm not a biologist, so I'm probably way confused here,
> but it seems to me there is a gray area (ORFs?).
>
> So I guess there are several competing implementations.
>
> 1. SEQUENCE BASED, FEATURES REPRESENT DATA TRACKS
>
> "Chromosome 1" : Sequence
> "AA504327" : SubSequence
> "RAT2N" : Feature
> <anon> : Annotation
> "Value" => 2.34312
> "RAT2_MEAN": Feature
> <anon> : Annotation
> "Value" => 1.8342
> "AA432030" : SubSequence
> ...
>
> 2. SEQUENCE BASED, ANNOTATIONS REPRESENT DATA TRACKS
>
> "Chromosome 1" : Sequence
> "AA504327" : SubSequence
> "Microarray Experiment B2341v" : Feature
> <anon> : Annotation
> "RAT2N" => 2.34312
> "RAT2_MEAN" => 1.8342
> "AA432030" : SubSequence
> ...
>
> 3. FEATURE BASED, SUB-FEATURES REPRESENT DATA TRACKS
>
> (Very similar to #1, except using Feature instead of SubSequence.)
>
> "Chromosome 1" : Sequence
> "AA504327" : Feature
> "RAT2N" : Feature
> <anon> : Annotation
> "Value" => 2.34312
> "RAT2_MEAN": Feature
> <anon> : Annotation
> "Value" => 1.8342
> "AA432030" : SubSequence
> ...
>
> 4. FEATURE BASED, ANNOTATIONS REPRESENT DATA TRACKS
>
> (Very similar to #2, except using Feature instead of SubSequence.)
>
> "Chromosome 1" : Sequence
> "AA504327" : Feature
> "Microarray Experiment B2341v" : Feature
> <anon> : Annotation
> "RAT2N" => 2.34312
> "RAT2_MEAN" => 1.8342
> "AA432030" : SubSequence
> ...
>
> Thanks a lot again & peace,
>
> Ihab
>
> --
> Ihab A.B. Awad <ihab at stanford.edu>
> Snr Scientific Programmer, Dept of Genetics
>
> ------------------------------
>
> Message: 2
> Date: Wed, 10 Sep 2003 09:06:00 -0700
> From: "Gabriel del Rio Guerra" <gdelrio at buckinstitute.org>
> Subject: [Biojava-l] Reformating multiple sequence alignments
> To: <biojava-l at biojava.org>
> Message-ID:
> <4AA34A707932424EBE2D973764D226A91B98D7 at inverness.buckcenter.org>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi,
>
> I am interesting in using or developing a program to inter-convert
> different sequence alignments formats. In particular I want to translate
> HSSP alignments into MSF format. Does anyone knows if this is possible
> with Biojava at this point?
>
> Thanks,
>
> Gabriel
>
> -----Original Message-----
> From: Kenny Yu [mailto:kyu at biodiscovery.com]
> Sent: Wednesday, April 30, 2003 8:45 AM
> To: biojava-l at biojava.org
> Subject: [Biojava-l] unified consolidated annotation system
>
> Both biosql and biodas are concerned with retrieving annotation on the
> basis of known features. They are not optimized for large-scale
> cross-system queries and data mining on the basis of annotations. I am
> interested in consolidating annotations from various sources (such as
> genbank and unigene) into a unified form on which I can run queries like
> "find features that are linked to DNA repair function in human but not
> in mouse" and "find feature whose molecular functions overlap with
> those of Accession Number nnnn". Similar mechanisms may exist in LION's
> SRS (http://www.lionbioscience.com/solutions/products/srs/relational).
> What I am contemplating is essentially a data warehouse for annotations.
> I'll borrow data warehouse and OLAP techniques in the design. It's a
> hybrid of relational, nested-relational and multidimensional database.
> Feature entity, or gene, and annotation are the dimensions. It models
> textual values as well as binary objects such as pathway diagrams as
> annot!
> ation values. I intend to make my work open-source and would call it
> UCAsql. Currently the schema design, with some documentation and java
> API, is available to anyone upon request by email to myself at
> kyu at biodiscovery.com.
>
> _______________________________________________
> Biojava-l mailing list - Biojava-l at biojava.org
> http://biojava.org/mailman/listinfo/biojava-l
>
> ------------------------------
>
> Message: 3
> Date: Wed, 10 Sep 2003 20:13:41 +0200
> From: " charles.girardot at libertysurf.fr "
> <charles.girardot at libertysurf.fr>
> Subject: [Biojava-l] Parsing location from gbk files
> To: " biojava-l " <biojava-l at biojava.org>
> Message-ID: <HL0FYT$52C54620864A877D0DA8660E2F2B1626 at tiscali.fr>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi,
>
> I am using biojava to extract mRNA, CDS and gene boundaries from genbank annotation files (e.g., "hs_chr19.gbs" from ftp://ftp.ncbi.nih.gov/genomes/H_sapiens/CHR_19/).
> I've just switched from the biojava 1.22 to latest 1.3 release (biojava-1.30-jdk14.jar and bytecode-0.92.jar)
> and I get lots of exception (see below, the peace of code generating this follows as well). Don't known if it's important but ".gbs" files do not hold the contig sequences but only annotations.
>
> Nota: I am using the jdk 1.4.1.
>
> Thanks for your help.
>
> Charles Girardot
>
> =======================================
> java.lang.IllegalArgumentException: Location 59817 is outside 1..0
> at org.biojava.bio.seq.impl.SimpleFeature.<init>(SimpleFeature.java:306)
> at org.biojava.bio.seq.impl.SimpleStrandedFeature.<init>(SimpleStrandedFeature.java:74)
> at sun.reflect.GeneratedConstructorAccessor1.newInstance(Unknown Source)
> at sun.reflect.DelegatingConstructorAccessorImpl.newInstance(DelegatingConstructorAccessorImpl.java:27)
> at java.lang.reflect.Constructor.newInstance(Constructor.java:274)
> at org.biojava.bio.seq.SimpleFeatureRealizer$TemplateImpl.realize(SimpleFeatureRealizer.java:138)
> rethrown as org.biojava.bio.BioException: Couldn't realize feature
> at org.biojava.bio.seq.SimpleFeatureRealizer$TemplateImpl.realize(SimpleFeatureRealizer.java:144)
> at org.biojava.bio.seq.SimpleFeatureRealizer.realizeFeature(SimpleFeatureRealizer.java:94)
> at org.biojava.bio.seq.impl.SimpleSequence.realizeFeature(SimpleSequence.java:198)
> at org.biojava.bio.seq.impl.SimpleSequence.createFeature(SimpleSequence.java:204)
> at org.biojava.bio.seq.io.SequenceBuilderBase.makeSequence(SequenceBuilderBase.java:168)
> at org.biojava.bio.seq.io.SmartSequenceBuilder.makeSequence(SmartSequenceBuilder.java:87)
> at org.biojava.bio.seq.io.SequenceBuilderFilter.makeSequence(SequenceBuilderFilter.java:98)
> at org.biojava.bio.seq.io.StreamReader.nextSequence(StreamReader.java:101)
> at org.gc.test.GenbankParserTest.main(GenbankParserTest.java:34)
>
>
> ==========================================
>
> package org.gc.test;
>
> import java.io.BufferedReader;
> import java.io.File;
> import java.io.FileReader;
>
> import org.biojava.bio.seq.Sequence;
> import org.biojava.bio.seq.SequenceIterator;
> import org.biojava.bio.seq.io.SeqIOTools;
>
> /**
> * @author unknown
> *
> * To change the template for this generated type comment go to
> * Window>Preferences>Java>Code Generation>Code and Comments
> */
> public class GenbankParserTest {
>
> public static void main(String[] args) {
> System.out.println("Main starts ...");
> try {
>
> File f = new File("D:\\Travail\\data\\Hs_chr19.gbs");
> BufferedReader bf = new BufferedReader(new FileReader(f));
> SequenceIterator it = SeqIOTools.readGenbank(bf);
> while (it.hasNext()) {
> System.out.println("getting next seq...");
> Sequence s = it.nextSequence();
> }
> } catch (Exception e) {
> System.out.println(e.getStackTrace());
> }
> System.out.println("End of main");
> }
> }
>
> ********** L'ADSL A 20 EUR/MOIS**********
> Avec Tiscali, l'ADSL est à 20 EUR/mois. Vous pourrez chercher longtemps avant de trouver moins cher !
> Pour profiter de cette offre exceptionnelle, cliquez ici : http://register.tiscali.fr/adsl/
> Offre soumise à conditions.
>
> ------------------------------
>
> Message: 4
> Date: Thu, 11 Sep 2003 09:40:34 +1200
> From: "Schreiber, Mark" <mark.schreiber at agresearch.co.nz>
> Subject: RE: [Biojava-l] Reformating multiple sequence alignments
> To: "Gabriel del Rio Guerra" <gdelrio at buckinstitute.org>,
> <biojava-l at biojava.org>
> Message-ID:
> <AF026AF0FF4B054590228FD1F1DE5165011BA68A at inbox.agresearch.co.nz>
> Content-Type: text/plain; charset="UTF-8"
>
> Hi -
>
> I'm not too familiar with what you are trying to do but...
>
> A lot of the biojava Alignment API can be found in org.biojava.bio.alignment parsers for reading and writing different formats are in org.biojava.bio.seq.io.* SeqIOTools is especially useful.
>
> Hope this helps:
>
> - Mark
>
>
> -----Original Message-----
> From: Gabriel del Rio Guerra [mailto:gdelrio at buckinstitute.org]
> Sent: Thu 11/09/2003 4:06 a.m.
> To: biojava-l at biojava.org
> Cc:
> Subject: [Biojava-l] Reformating multiple sequence alignments
>
>
>
> Hi,
>
> I am interesting in using or developing a program to inter-convert
> different sequence alignments formats. In particular I want to translate
> HSSP alignments into MSF format. Does anyone knows if this is possible
> with Biojava at this point?
>
> Thanks,
>
> Gabriel
>
> -----Original Message-----
> From: Kenny Yu [mailto:kyu at biodiscovery.com]
> Sent: Wednesday, April 30, 2003 8:45 AM
> To: biojava-l at biojava.org
> Subject: [Biojava-l] unified consolidated annotation system
>
> Both biosql and biodas are concerned with retrieving annotation on the
> basis of known features. They are not optimized for large-scale
> cross-system queries and data mining on the basis of annotations. I am
> interested in consolidating annotations from various sources (such as
> genbank and unigene) into a unified form on which I can run queries like
> "find features that are linked to DNA repair function in human but not
> in mouse" and "find feature whose molecular functions overlap with
> those of Accession Number nnnn". Similar mechanisms may exist in LION's
> SRS (http://www.lionbioscience.com/solutions/products/srs/relational).
> What I am contemplating is essentially a data warehouse for annotations.
> I'll borrow data warehouse and OLAP techniques in the design. It's a
> hybrid of relational, nested-relational and multidimensional database.
> Feature entity, or gene, and annotation are the dimensions. It models
> textual values as well as binary objects such as pathway diagrams as
> annot!
> ation values. I intend to make my work open-source and would call it
> UCAsql. Currently the schema design, with some documentation and java
> API, is available to anyone upon request by email to myself at
> kyu at biodiscovery.com.
>
> _______________________________________________
> Biojava-l mailing list - Biojava-l at biojava.org
> http://biojava.org/mailman/listinfo/biojava-l
>
> _______________________________________________
> Biojava-l mailing list - Biojava-l at biojava.org
> http://biojava.org/mailman/listinfo/biojava-l
>
>
> =======================================================================
> Attention: The information contained in this message and/or attachments
> from AgResearch Limited is intended only for the persons or entities
> to which it is addressed and may contain confidential and/or privileged
> material. Any review, retransmission, dissemination or other use of, or
> taking of any action in reliance upon, this information by persons or
> entities other than the intended recipients is prohibited by AgResearch
> Limited. If you have received this message in error, please notify the
> sender immediately.
> =======================================================================
>
> ------------------------------
>
> Message: 5
> Date: Thu, 11 Sep 2003 09:44:04 +1200
> From: "Schreiber, Mark" <mark.schreiber at agresearch.co.nz>
> Subject: RE: [Biojava-l] Parsing location from gbk files
> To: <charles.girardot at libertysurf.fr>, "biojava-l"
> <biojava-l at biojava.org>
> Message-ID:
> <AF026AF0FF4B054590228FD1F1DE5165011BA68C at inbox.agresearch.co.nz>
> Content-Type: text/plain; charset="UTF-8"
>
> Hi -
>
> This is a recurring problem as more files are being deposited that contain only references to other contigs but contain annotation and features.
>
> A model where a DummySequence of appropriate length is instantiated if the contig files are not available may be useful. Possibley a GBS parser would be useful? Has anyone hacked out something like this?
>
> - Mark
>
> -----Original Message-----
> From: charles.girardot at libertysurf.fr [mailto:charles.girardot at libertysurf.fr]
> Sent: Thu 11/09/2003 6:13 a.m.
> To: biojava-l
> Cc:
> Subject: [Biojava-l] Parsing location from gbk files
>
>
>
> Hi,
>
> I am using biojava to extract mRNA, CDS and gene boundaries from genbank annotation files (e.g., "hs_chr19.gbs" from ftp://ftp.ncbi.nih.gov/genomes/H_sapiens/CHR_19/).
> I've just switched from the biojava 1.22 to latest 1.3 release (biojava-1.30-jdk14.jar and bytecode-0.92.jar)
> and I get lots of exception (see below, the peace of code generating this follows as well). Don't known if it's important but ".gbs" files do not hold the contig sequences but only annotations.
>
> Nota: I am using the jdk 1.4.1.
>
> Thanks for your help.
>
> Charles Girardot
>
>
> =======================================
> java.lang.IllegalArgumentException: Location 59817 is outside 1..0
> at org.biojava.bio.seq.impl.SimpleFeature.<init>(SimpleFeature.java:306)
> at org.biojava.bio.seq.impl.SimpleStrandedFeature.<init>(SimpleStrandedFeature.java:74)
> at sun.reflect.GeneratedConstructorAccessor1.newInstance(Unknown Source)
> at sun.reflect.DelegatingConstructorAccessorImpl.newInstance(DelegatingConstructorAccessorImpl.java:27)
> at java.lang.reflect.Constructor.newInstance(Constructor.java:274)
> at org.biojava.bio.seq.SimpleFeatureRealizer$TemplateImpl.realize(SimpleFeatureRealizer.java:138)
> rethrown as org.biojava.bio.BioException: Couldn't realize feature
> at org.biojava.bio.seq.SimpleFeatureRealizer$TemplateImpl.realize(SimpleFeatureRealizer.java:144)
> at org.biojava.bio.seq.SimpleFeatureRealizer.realizeFeature(SimpleFeatureRealizer.java:94)
> at org.biojava.bio.seq.impl.SimpleSequence.realizeFeature(SimpleSequence.java:198)
> at org.biojava.bio.seq.impl.SimpleSequence.createFeature(SimpleSequence.java:204)
> at org.biojava.bio.seq.io.SequenceBuilderBase.makeSequence(SequenceBuilderBase.java:168)
> at org.biojava.bio.seq.io.SmartSequenceBuilder.makeSequence(SmartSequenceBuilder.java:87)
> at org.biojava.bio.seq.io.SequenceBuilderFilter.makeSequence(SequenceBuilderFilter.java:98)
> at org.biojava.bio.seq.io.StreamReader.nextSequence(StreamReader.java:101)
> at org.gc.test.GenbankParserTest.main(GenbankParserTest.java:34)
>
>
>
> ==========================================
>
> package org.gc.test;
>
> import java.io.BufferedReader;
> import java.io.File;
> import java.io.FileReader;
>
> import org.biojava.bio.seq.Sequence;
> import org.biojava.bio.seq.SequenceIterator;
> import org.biojava.bio.seq.io.SeqIOTools;
>
> /**
> * @author unknown
> *
> * To change the template for this generated type comment go to
> * Window>Preferences>Java>Code Generation>Code and Comments
> */
> public class GenbankParserTest {
>
> public static void main(String[] args) {
> System.out.println("Main starts ...");
> try {
>
> File f = new File("D:\\Travail\\data\\Hs_chr19.gbs");
> BufferedReader bf = new BufferedReader(new FileReader(f));
> SequenceIterator it = SeqIOTools.readGenbank(bf);
> while (it.hasNext()) {
> System.out.println("getting next seq...");
> Sequence s = it.nextSequence();
> }
> } catch (Exception e) {
> System.out.println(e.getStackTrace());
> }
> System.out.println("End of main");
> }
> }
>
>
> ********** L'ADSL A 20 EUR/MOIS**********
> Avec Tiscali, l'ADSL est à 20 EUR/mois. Vous pourrez chercher longtemps avant de trouver moins cher !
> Pour profiter de cette offre exceptionnelle, cliquez ici : http://register.tiscali.fr/adsl/
> Offre soumise à conditions.
>
>
>
> _______________________________________________
> Biojava-l mailing list - Biojava-l at biojava.org
> http://biojava.org/mailman/listinfo/biojava-l
>
>
> =======================================================================
> Attention: The information contained in this message and/or attachments
> from AgResearch Limited is intended only for the persons or entities
> to which it is addressed and may contain confidential and/or privileged
> material. Any review, retransmission, dissemination or other use of, or
> taking of any action in reliance upon, this information by persons or
> entities other than the intended recipients is prohibited by AgResearch
> Limited. If you have received this message in error, please notify the
> sender immediately.
> =======================================================================
>
> ------------------------------
>
> Message: 6
> Date: Thu, 11 Sep 2003 10:58:16 +0100
> From: Matthew Pocock <matthew_pocock at yahoo.co.uk>
> Subject: Re: [Biojava-l] Parsing location from gbk files
> To: "Schreiber, Mark" <mark.schreiber at agresearch.co.nz>
> Cc: biojava-l <biojava-l at biojava.org>
> Message-ID: <3F604738.6080504 at yahoo.co.uk>
> Content-Type: text/plain; charset=UTF-8; format=flowed
>
> Schreiber, Mark wrote:
>
> >Hi -
> >
> >This is a recurring problem as more files are being deposited that contain only references to other contigs but contain annotation and features.
> >
> >A model where a DummySequence of appropriate length is instantiated if the contig files are not available may be useful. Possibley a GBS parser would be useful? Has anyone hacked out something like this?
> >
> >- Mark
> >
> >
> It should be possible to create a dummy sequence of the right length and
> then populate the feature table with component features for the assembly
> info. Perhaps if we wrote a ComponentFeature that held an LSID - then
> tries to resolve itself on demand via the LSID framework?
>
> I don't have the time right now, but somebody should get keen.
>
> Matthew
>
> > -----Original Message-----
> > From: charles.girardot at libertysurf.fr [mailto:charles.girardot at libertysurf.fr]
> > Sent: Thu 11/09/2003 6:13 a.m.
> > To: biojava-l
> > Cc:
> > Subject: [Biojava-l] Parsing location from gbk files
> >
> >
> >
> > Hi,
> >
> > I am using biojava to extract mRNA, CDS and gene boundaries from genbank annotation files (e.g., "hs_chr19.gbs" from ftp://ftp.ncbi.nih.gov/genomes/H_sapiens/CHR_19/).
> > I've just switched from the biojava 1.22 to latest 1.3 release (biojava-1.30-jdk14.jar and bytecode-0.92.jar)
> > and I get lots of exception (see below, the peace of code generating this follows as well). Don't known if it's important but ".gbs" files do not hold the contig sequences but only annotations.
> >
> > Nota: I am using the jdk 1.4.1.
> >
> > Thanks for your help.
> >
> > Charles Girardot
> >
> >
> > =======================================
> > java.lang.IllegalArgumentException: Location 59817 is outside 1..0
> > at org.biojava.bio.seq.impl.SimpleFeature.<init>(SimpleFeature.java:306)
> > at org.biojava.bio.seq.impl.SimpleStrandedFeature.<init>(SimpleStrandedFeature.java:74)
> > at sun.reflect.GeneratedConstructorAccessor1.newInstance(Unknown Source)
> > at sun.reflect.DelegatingConstructorAccessorImpl.newInstance(DelegatingConstructorAccessorImpl.java:27)
> > at java.lang.reflect.Constructor.newInstance(Constructor.java:274)
> > at org.biojava.bio.seq.SimpleFeatureRealizer$TemplateImpl.realize(SimpleFeatureRealizer.java:138)
> > rethrown as org.biojava.bio.BioException: Couldn't realize feature
> > at org.biojava.bio.seq.SimpleFeatureRealizer$TemplateImpl.realize(SimpleFeatureRealizer.java:144)
> > at org.biojava.bio.seq.SimpleFeatureRealizer.realizeFeature(SimpleFeatureRealizer.java:94)
> > at org.biojava.bio.seq.impl.SimpleSequence.realizeFeature(SimpleSequence.java:198)
> > at org.biojava.bio.seq.impl.SimpleSequence.createFeature(SimpleSequence.java:204)
> > at org.biojava.bio.seq.io.SequenceBuilderBase.makeSequence(SequenceBuilderBase.java:168)
> > at org.biojava.bio.seq.io.SmartSequenceBuilder.makeSequence(SmartSequenceBuilder.java:87)
> > at org.biojava.bio.seq.io.SequenceBuilderFilter.makeSequence(SequenceBuilderFilter.java:98)
> > at org.biojava.bio.seq.io.StreamReader.nextSequence(StreamReader.java:101)
> > at org.gc.test.GenbankParserTest.main(GenbankParserTest.java:34)
> >
> >
> >
> > ==========================================
> >
> > package org.gc.test;
> >
> > import java.io.BufferedReader;
> > import java.io.File;
> > import java.io.FileReader;
> >
> > import org.biojava.bio.seq.Sequence;
> > import org.biojava.bio.seq.SequenceIterator;
> > import org.biojava.bio.seq.io.SeqIOTools;
> >
> > /**
> > * @author unknown
> > *
> > * To change the template for this generated type comment go to
> > * Window>Preferences>Java>Code Generation>Code and Comments
> > */
> > public class GenbankParserTest {
> >
> > public static void main(String[] args) {
> > System.out.println("Main starts ...");
> > try {
> >
> > File f = new File("D:\\Travail\\data\\Hs_chr19.gbs");
> > BufferedReader bf = new BufferedReader(new FileReader(f));
> > SequenceIterator it = SeqIOTools.readGenbank(bf);
> > while (it.hasNext()) {
> > System.out.println("getting next seq...");
> > Sequence s = it.nextSequence();
> > }
> > } catch (Exception e) {
> > System.out.println(e.getStackTrace());
> > }
> > System.out.println("End of main");
> > }
> > }
> >
> >
> > ********** L'ADSL A 20 EUR/MOIS**********
> > Avec Tiscali, l'ADSL est à 20 EUR/mois. Vous pourrez chercher longtemps avant de trouver moins cher !
> > Pour profiter de cette offre exceptionnelle, cliquez ici : http://register.tiscali.fr/adsl/
> > Offre soumise à conditions.
> >
> >
> >
> > _______________________________________________
> > Biojava-l mailing list - Biojava-l at biojava.org
> > http://biojava.org/mailman/listinfo/biojava-l
> >
> >
> >
> >=======================================================================
> >Attention: The information contained in this message and/or attachments
> >from AgResearch Limited is intended only for the persons or entities
> >to which it is addressed and may contain confidential and/or privileged
> >material. Any review, retransmission, dissemination or other use of, or
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