[Biojava-dev] Percentage similarity
Cleveland, John S
ClevelandJ at BATTELLE.ORG
Tue Sep 26 14:42:57 UTC 2006
Does anyone know how to retrieve the percentage similarity from a BLAST
result using BioJava?
This field is not available from SeqSimilaritySearchSubHit.
SeqSimilaritySearchSubHit does have the getEValue() and getScore()
methods, so I was a little confused about not finding the "percentage
identity" and "percentage similarity" fields. I followed the directions
in http://biojava.org/wiki/BioJava:CookBook:Blast:Echo, but again the
percentage similarity does not seem to be getting parsed by the
BlastLikeSaxParser. Here is the result of the aforementioned code:
startHit()
HitProp: subjectSequenceLength: 299
HitProp: subjectId: lcd|5392-AAA98259
HitProp: subjectDescription:
startSubHit()
SubHitProp: score: 24.6
SubHitProp: expectValue: 6.9
SubHitProp: numberOfIdentities: 14
SubHitProp: alignmentSize: 42
SubHitProp: percentageIdentity: 33
SubHitProp: numberOfIdentities: 14
SubHitProp: numberOfPositives: 23
SubHitProp: queryFrame: plus1
SubHitProp: querySequenceStart: 928
SubHitProp: querySequenceEnd: 1047
SubHitProp: querySequence:
TKDGKTQEWEMDNPGN--DFMTGSKDTYTFKLKDENLKIDDI
SubHitProp: subjectSequenceStart: 126
SubHitProp: subjectSequenceEnd: 167
SubHitProp: subjectSequence:
TDDGKIREYELPNKGSYPSFITLGSDNALWFTENQNNAIGRI
endSubHit()
endHit()
Thanks,
John Cleveland
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