[EMBOSS] ABI to FASTQ with seqret

Peter biopython at maubp.freeserve.co.uk
Thu Jul 22 16:45:47 UTC 2010


On Thu, Jul 22, 2010 at 5:33 PM, Tom Keller <kellert at ohsu.edu> wrote:
> Greetings,
> The latest versions of the ABI basecaller does indeed give quality scores.

I suspect the problem is my ABI files were not created using the latest ABI
basecaller then. Do you have any more details (e.g. which version)?

I've sent a couple of *.ab1 files off list to Peter Rice to confirm they really
don't have quality scores.

Tomorrow I will try and find out who to contact locally about the base calling,
and what version of the base caller they have.

Peter



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