[EMBOSS] Trim polyA from fastq files

Peter Rice pmr at ebi.ac.uk
Thu Oct 1 12:09:48 UTC 2009


On 01/10/09 12:33, michael watson (IAH-C) wrote:
> Hello
>
> I have some Illumina reads which have varying lengths of polyAs at the ends.  I want to trim these off.  The data is in fastq format.
>
> Is this possible in EMBOSS?  I have 6.0.1 and I tried trimest but it couldn't read my fastq file

Yes, but you need EMBOSS 6.1.0 to support FASTQ input.

There are also a couple of patches that are needed for some FASTQ files.

regards,

Peter Rice



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