[EMBOSS] FASTQ records with no sequence?

Peter biopython at maubp.freeserve.co.uk
Thu Jul 30 15:19:24 UTC 2009


On Thu, Jul 30, 2009 at 4:09 PM, Peter Rice<pmr at ebi.ac.uk> wrote:
>
> Peter C. wrote:
>
>> As we are recommending no line wrapping on output this means
>> typical FASTQ records would be four lines - so doing the same
>> makes sense here too.
>
> I vote for 4 lines on output.

If we want to allow zero length sequences, then yes, I would also
vote for the 4 line output (i.e. blank lines for the sequence and
the quality string).

> It should be possible to allow zero lines on input depending on
> where the '+' check is.

Yes, I'm pretty sure a parser could cope with any of the zero length
sequence FASTQ examples I gave.

Peter



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