[EMBOSS] inosine in nucleotide sequence databases

Wells, Isabelle isabelle.wells at roche.com
Tue Aug 18 08:25:41 UTC 2009

Hi all,
Can emboss handle inosine in nucleotide sequences? We have a nucleotide file in embl format where some sequences contain inosine. Dbiflat doesn't seem to index the database properly although no error message was given and those inosine containing sequences cannot be retrieved with seqret. Any suggestions on what we could do apart from replacing inosine by X or N?
Many thanks,
Isabelle Wells

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