[EMBOSS] MW in "Digest" -EMBOSS

Mehrotra, Sudeep sum732 at mail.usask.ca
Tue Aug 14 20:46:03 UTC 2007


Hello,

I am trying to figure out how molecular weight of the peptide fragments in
EMBOSS
"DIGEST" is calculated?

For example: given fragment AEK, "DIGEST" returns a MW = 346.378.

Now if i look at the residual masses(monoisotopic) of A E K they are 71.03711,
129.04259, 128.09496, respectively, these dont add up to 346.378. I tried using
Average mass, as well as normal mw of AA in question, but i am not to get a MW
of
346.378.
It would be very helpful if you can explain how MW is calculated in
EMBOSS-DIGEST.

Thanking You
Sudeep




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