[EMBOSS] eprimer3

[Gary Williams, Tel 01223 494522]

The 23 input sequence limitation of eprimer3 will be fixed in the next
release.
In the meantime, you could get a copy of the fixed program from:
ftp://ftp.rfcgr.mrc.ac.uk/pub/EMBOSS/patchfiles/eprimer3.*

Regards,
Gary

Henrikki Almusa wrote:
> [EMBOSS] eprimer3: max input file number?
> Date: 21.10.2003 19:41
> From: Iain Drummond <idrummon at receptor.mgh.harvard.edu>
> 
> I have run into a limitation using eprimer3 as an interface for primer3. It
> seems that eprimer3 will accept up to 23 sequences (in a single fasta
> formatted text file) but not 24 or more; with 24 or more sequences, eprimer3
> and primer3_core will just hang as ³sleeping² processes and never finish. My
> goal is to automate genomic sequence analysis (zebrafish) in order to
> generate new, potentially useful polymorphic markers from dinucleotide
> repeats. eprimer3 is the last step where a long list of ~500 bp sequences
> are sent to primer3 to make primers.


-- 
Gary Williams
MRC Rosalind Franklin Centre for Genomics Research
Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SB, UK
Tel: +44 1223 494522			Fax: +44 1223 494512
E-mail: gwilliam at rfcgr.mrc.ac.uk	Web: http://www.rfcgr.mrc.ac.uk