[EMBOSS] esim4 man page
Derek Gatherer
d.gatherer at vir.gla.ac.uk
Wed Mar 17 16:34:31 UTC 2004
Hi
Is there an esim4 man page?
http://www.hgmp.mrc.ac.uk/Software/EMBOSS/Apps/index.html
The rest of EMBASSY is above, but not esim4. The original sim4 has a quick
help list, but I wondered if there is any extra functionality in the
EMBASSY version.
Cheers
Derek
> sim4
sim4 seq1 seq2_db [[WXKCRDHAPNBS]=]
W - word size. (W=12)
X - value for terminating word extensions. (X=12)
K - MSP score threshold for the first pass. (e.g., K=16)
C - MSP score threshold for the second pass. (e.g., C=12)
R - direction of search; 0 - search the '+' (direct) strand only;
1 - search the '-' strand only; 2 - search both strands and
report the best match. (R=2)
D - bound for the range of diagonals within consecutive msps in an
exon. (D=10)
H - weight factor for MSP scores in relinking. (H=500)
A - output format: exon endpoints only (A=0), alignment text (A=1),
alignment in lav (block) format (A=2), or both exon endpoints
and alignment text (A=3, A=4). If complement match, A=0,1,2,3
give direct positions in the long sequence and complement
positions in the short sequence. A=4 gives direct positions in
the first sequence, regardless of the relative lengths.
A=5 prints the exon and CDS coordinates (the latter, if known)
in the `exon file' format required by PipMaker. To be used
with full-length mRNA sequences.
P - if not 0, remove poly-A tails; report coordinates in the
'+' (direct) strand for complement matches; use lav alignment
headers in all display options. (P=0)
N - accuracy of sequences (non-zero for highly accurate). (N=0)
B - if 0, dis-allow ambiguity codes (other than N and X) in the
sequence data. (B=1)
S - coding region specification (available only with A=5);
format: S=n1..n2
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