'dan' related questions

[Anand Rathi]

Hi,

	I am using 'dan' to plot the melting temperature of a DNA sequence(~16
kbp). What I have observed is as I decrease the Window size, the melting
temperature also changes. When I make the window size 2, I get melting
temperature of the range -150 degrees! I checked the original paper by
Breslauer et al and also recent one by Allawi & SantaLucia. Both of them
have no mention of the melting temperature being dependent on the
length. Could someone please explain ?

Thanks,
Anand Rathi,
Virginia Bioinformatics Institute