eprimer3...broken pipe?

[Gary Williams, Tel 01223 494522]

>From the eprimer3 documentation:

The Whitehead program must be set up and on the path in order for
eprimer3 to find and run it. 

The Whitehead Institute program that is run by this program is available
from:
http://www-genome.wi.mit.edu/genome_software/other/primer3.html 
(Then see the link 'Get release 0.9') 

The version that is run by this program is 3.0.9 currently available
from:
http://www-genome.wi.mit.edu/ftp/distribution/software/primer3_0_9_test.tar.gz 

Gary


Burke Squires wrote:
> 
> I have tried to install various version of emboss and when I try and run
> eprimer3 I get the following message:
> 
> [loopback:bioinfo/emboss-2.4.1/emboss] bsquires% eprimer3
> Picks PCR primers and hybridization oligos
> Input sequence(s): /bioinfo/fragments.fa
> Output file [tpe-v_a.eprimer3]: /bioinfo/fa.out
> 
>    EMBOSS An error in eprimer3.c at line 317:
> The program 'primer3_core' must be on the path.
> It is part of the 'primer3' package,
> available from the Whitehead Institute.
> See: http://www-genome.wi.mit.edu/
> Broken pipe
> 
> Does anybody know how to fix this?
> 
> Thanks!
> 
> Burke Squires
> 
> --
> Burke Squires
> Bioinformatics
> MacroGenics, Inc.
> 2600 Stemmons Freeway, Suite 210
> Dallas, TX 75235 USA
> Work: 214-634-3000 X224
> Squiresb @ macrogenics.com (Please remove spaces to use)
> www.macrogenics.com
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-- 
Gary Williams               Tel: +44 1223 494522  Fax: +44 1223 494512
mailto:G.Williams at hgmp.mrc.ac.uk            http://www.hgmp.mrc.ac.uk/
Bioinformatics,MRC HGMP Resource Centre,Hinxton,Cambridge, CB10 1SB,UK