eprimer3...broken pipe?

Gary Williams, Tel 01223 494522 gwilliam at hgmp.mrc.ac.uk
Wed Oct 9 08:28:08 UTC 2002

>From the eprimer3 documentation:

The Whitehead program must be set up and on the path in order for
eprimer3 to find and run it. 

The Whitehead Institute program that is run by this program is available
(Then see the link 'Get release 0.9') 

The version that is run by this program is 3.0.9 currently available


Burke Squires wrote:
> I have tried to install various version of emboss and when I try and run
> eprimer3 I get the following message:
> [loopback:bioinfo/emboss-2.4.1/emboss] bsquires% eprimer3
> Picks PCR primers and hybridization oligos
> Input sequence(s): /bioinfo/fragments.fa
> Output file [tpe-v_a.eprimer3]: /bioinfo/fa.out
>    EMBOSS An error in eprimer3.c at line 317:
> The program 'primer3_core' must be on the path.
> It is part of the 'primer3' package,
> available from the Whitehead Institute.
> See: http://www-genome.wi.mit.edu/
> Broken pipe
> Does anybody know how to fix this?
> Thanks!
> Burke Squires
> --
> Burke Squires
> Bioinformatics
> MacroGenics, Inc.
> 2600 Stemmons Freeway, Suite 210
> Dallas, TX 75235 USA
> Work: 214-634-3000 X224
> Squiresb @ macrogenics.com (Please remove spaces to use)
> www.macrogenics.com
> ----------------------------------------------------------------------------
> This e-mail and any attachments may be confidential or legally privileged.
> If you received this message in error or are not the intended recipient, you
> should destroy the e-mail message and any attachments or copies, and you are
> prohibited from retaining, distributing, disclosing or using any information
> contained herein.  Please inform us of the erroneous delivery by return
> e-mail.
> Thank you for your cooperation.

Gary Williams               Tel: +44 1223 494522  Fax: +44 1223 494512
mailto:G.Williams at hgmp.mrc.ac.uk            http://www.hgmp.mrc.ac.uk/
Bioinformatics,MRC HGMP Resource Centre,Hinxton,Cambridge, CB10 1SB,UK

More information about the EMBOSS mailing list