test sequence

ableasby at hgmp.mrc.ac.uk ableasby at hgmp.mrc.ac.uk
Fri Nov 1 20:21:29 UTC 2002

There is probably not a problem. EMBOSS only reports one
isoschizomer for cases where several REs have the same cut
site. If the  -preferred   switch is given to these
programs then the more easily available of the isoschizomers
will be reported. This is controlled by the file:


where, for each RE, you can specify which isoschizomer should be

So, first try adding -preferred. If you just want to search for
a particular set of enzymes they can be given as a comma-separated
list using the -enzymes qualifier   e.g. -enzymes "ecori bamhi"


Alan Bleasby

PS: NEB supply an emboss-format set of files which are just the most
    common REs. You can rename them (e.g. to embossre.enz/ref/sup)
    and overwrite your current set in the emboss REBASE directory.

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