[BioRuby] Melting Temperature

Rob Syme rob.syme at gmail.com
Thu Oct 9 15:08:49 UTC 2014


Hi Pjotr

I was looking as well. The only repo is https://github.com/shevegen/bioroebe,
but it is empty :(

-r

On Thu, Oct 9, 2014 at 11:04 AM, Pjotr Prins <pjotr.public14 at thebird.nl>
wrote:

> Github repository? Would be nice to add to biogems.info.
>
> Pj.
>
> On Thu, Oct 09, 2014 at 10:35:34AM -0400, Rob Syme wrote:
> > Hi David
> >
> > The 'bioroebe' gem includes Tm calculation.
> >
> >     $ gem install bioroebe
> >     $ irb
> >
> >     > require 'bioroebe/calculate_melting_temperature'
> >     > seq = 'ATGCTGACGTATGCGATCGTA'
> >     > tm = CalculateMeltingTemperature.new
> >     > tm.set_input(seq)
> >     > tm.calculate_tm
> >     # ==> 65.32166985617363
> >
> > You can set oligo nucleotide concentration, Na+ conc and
> > formamide_concentration.
> >
> > The method isn't too complicated
> > <
> http://rubydoc.info/gems/bioroebe/0.0.44/CalculateMeltingTemperature:calculate_tm
> >
> >
> > Rob Syme
> >
> > On Thu, Oct 9, 2014 at 6:10 AM, McCreavy David <
> David.McCreavy at lhch.nhs.uk>
> > wrote:
> >
> > > Hi Guys,
> > >
> > > Where in the API will I find methods for calculating Tm?
> > >
> > > Kind Regards, David
> > >
> > >
> > >
> > >
> > >
> > >
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