Hi all, I tried my $seq = $gb->get_Seq_by_gi('162138530'); print $seq->seq."\n"; I am sure that the sequence whose GI is 162138530 does exist in the database (http://www.ncbi.nlm.nih.gov/protein/162138543). However, no sequences could be given. Has anyone encountered the similar problem? Thanks. Sincerely yours, Cacau