[Bioperl-l] converting to clustalw alignment format
Tao Zhu
bill_zt at sina.com
Tue Sep 18 09:28:19 UTC 2012
I have a DNA seq alignment in FASTA format:
> scry
TATAAAAACATTAAGTGCTACTGCACTGATATAATTGTTAAC
> soct
TACAAAAATTTCAAATGCTATTGTACTGATATCATTGTCGAC
> spom
TTTAAAAATGTAGAGTGTTATTGCACTAATTTAAGCATTGAC
> sjap
GAAAAAGAAATCGAGTGTTATTGTACAGACTTGATTGTAAAC
I changed it to CLUSTALW format using Bio::AlignIO
my $in = shift;
my $out = shift;
use Bio::AlignIO;
my $align_obj = Bio::AlignIO->new(-file=>$in,
-format=>'fasta')->next_aln;
my $writeio_obj = Bio::AlignIO->new(-file=>">$out", -format=>'clustalw');
$writeio_obj->write_aln($align_obj);
The result is:
scry/1-139 TATAAAAACATTAAGTGCTACTGCACTGATATAATTGTTAAC
soct/1-137 TACAAAAATTTCAAATGCTATTGTACTGATATCATTGTCGAC
spom/1-135 TTTAAAAATGTAGAGTGTTATTGCACTAATTTAAGCATTGAC
sjap/1-87 GAAAAAGAAATCGAGTGTTATTGTACAGACTTGATTGTAAAC
*** * * * ** ** ** ** * * * * **
I didn't want positions like "/1-139" to be shown, I just want the same
sequence name as original.
How should I do to eliminate positions like "/1-XXX"? Thank you!
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