[Bioperl-l] Bio::Tools::Run::StandAloneBlast can't find path to blastall

[mindlessbrain]

Hey all, 

I'm trying to run some code for StandAloneBLast in Windows Vista:

[code]
#!/usr/bin/perl 

use Bio::DB::SwissProt;
use Bio::Tools::Run::StandAloneBlast;

BEGIN
{
	$ENV{PATH}="D:/blast-2.2.23+/bin/:";
}

my $database = new Bio::DB::SwissProt;
my $query = $database->get_Seq_by_id('TAUD_ECOLI');

my $factory = Bio::Tools::Run::StandAloneBlast->new(
				'program'  => 'blastp',
				'database' => 'swissprot',
				 _READMETHOD => "Blast"
			 );
my $blast_report = $factory->blastall($query);
my $result = $blast_report->next_result;
while( my $hit = $result->next_hit()) {
    print "\thit name: ", $hit->name(), 
    " significance: ", $hit->significance(), "\n";
}
[/code]

I installed BLAST from the NCBI website. I get this when I run dir on the
bin:

D:\blast-2.2.23+\bin>dir
 Volume in drive D has no label.
 Volume Serial Number is 224C-0190

 Directory of D:\blast-2.2.23+\bin

05/03/2010  03:02 PM    <DIR>          .
05/03/2010  03:02 PM    <DIR>          ..
03/08/2010  11:09 PM         2,789,376 blastdbcheck.exe
03/08/2010  11:09 PM         4,009,984 blastdbcmd.exe
03/08/2010  11:09 PM         1,810,432 blastdb_aliastool.exe
03/08/2010  11:09 PM         6,225,920 blastn.exe
03/08/2010  11:09 PM         6,221,824 blastp.exe
03/08/2010  11:09 PM         6,213,632 blastx.exe
03/08/2010  11:09 PM         5,316,608 blast_formatter.exe
03/08/2010  11:09 PM         3,215,360 convert2blastmask.exe
03/08/2010  11:09 PM         3,211,264 dustmasker.exe
03/08/2010  11:09 PM            51,178 legacy_blast.pl
03/08/2010  11:09 PM         3,866,624 makeblastdb.exe
03/08/2010  11:09 PM         3,612,672 makembindex.exe
03/08/2010  11:09 PM         6,344,704 psiblast.exe
03/08/2010  11:09 PM         6,201,344 rpsblast.exe
03/08/2010  11:09 PM         6,205,440 rpstblastn.exe
03/08/2010  11:09 PM         3,608,576 segmasker.exe
03/08/2010  11:09 PM         6,320,128 tblastn.exe
03/08/2010  11:09 PM         6,209,536 tblastx.exe
03/08/2010  11:09 PM            10,010 update_blastdb.pl
03/08/2010  11:09 PM         3,530,752 windowmasker.exe
              20 File(s)     84,975,364 bytes
               2 Dir(s)  122,390,626,304 bytes free

I have an ncbi.ini file in my windows directory that contains:
[NCBI]
DATA=D:\blast-2.2.23+\data
[BLAST]
BLASTDB=D:\blast-2.2.23+\db

Here's what my environmental variables looks like:

http://old.nabble.com/file/p28434717/environmental%2Bvariables.jpg 

Help would be very, very appreciated!


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