[Bioperl-l] use primer3 to design primers with multiplesequences
Cui, Wenwu (NIH/NCI) [F]
cuiw at mail.nih.gov
Wed May 10 16:02:55 UTC 2006
'PRIMER_SEQUENCE_ID' is not a key in the Bio::Tools::Primer3 output
hash.
You can find all legal keys by "print keys %{$result1};"
There is one point I don't understand:
When I add these two lines into my code (line 49 in my
code)
my $key_PRIMER_SEQUENCE_ID='PRIMER_SEQUENCE_ID';
print
"$key_PRIMER_SEQUENCE_ID\t${$result1}{$key_PRIMER_SEQUENCE_ID}\n";
I don't get the PRIMER_SEQUENCE_ID. Perl complains it
and says "Use of uninitialized value in concatenation
(.) or string at primer3-3 line 49."
Li
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