[Bioperl-l] use primer3 to design primers with multiplesequences

Cui, Wenwu (NIH/NCI) [F] cuiw at mail.nih.gov
Wed May 10 16:02:55 UTC 2006


'PRIMER_SEQUENCE_ID' is not a key in the Bio::Tools::Primer3 output
hash.

You can find all legal keys by "print keys %{$result1};"



There is one point I don't understand:

When I add these two lines into my code (line 49 in my
code)

	   my $key_PRIMER_SEQUENCE_ID='PRIMER_SEQUENCE_ID';		
  	      
  print
"$key_PRIMER_SEQUENCE_ID\t${$result1}{$key_PRIMER_SEQUENCE_ID}\n";

I don't get the PRIMER_SEQUENCE_ID. Perl complains it
and says "Use of uninitialized value in concatenation
(.) or string at primer3-3 line 49."


Li
	   









More information about the Bioperl-l mailing list