[Bioperl-l] primer parameters using primer3

[chen li]

Hi Jason,

I add the line code   
$primer3->primer_product_size_range("490-510");
 to my script. But it doesn't work nor primer3
complains it.

Li

--- Jason Stajich <jason.stajich at duke.edu> wrote:

> I put up some info on the wiki (and I encourage
> other people to do  
> the same!)
>
http://bioperl.org/wiki/Module:Bio::Tools::Run::Primer3
> 
> Set the command line parameters by just calling a
> function of the  
> name of the parameter.  To get a list of the
> available options, this  
> perl code will report it to you:
> 
> # what are the arguments, and what do they mean?
>    my $args = $primer3->arguments;
> 
>    print "ARGUMENT\tMEANING\n";
>    foreach my $key (keys %{$args}) {print "$key\t",
> $$args{$key}, "\n"}
> 
> The info for PRODUCT_SIZE_RANGE is:
>    (size range list, default 100-300) space
> separated list of product  
> sizes eg <a>-<b> <x>-<y>
> 
> I believe you can set the PCR product size with
>    $primer3->primer_product_size_range("490-510");
> 
> -jason
> On May 7, 2006, at 3:34 AM, chen li wrote:
> 
> > Hi all,
> >
> > I use Bio::Tools::Run::Primer3 to design PCR
> primers.
> > I want to change some default values, for example,
> to
> > increase the PCR product size to 490-510 bp
> instead of
> > using the default value of 100-300 bp. What should
> I
> > do ?
> >
> >
> > Thanks,
> >
> > Li
> >
> > __________________________________________________
> > Do You Yahoo!?
> > Tired of spam?  Yahoo! Mail has the best spam
> protection around
> > http://mail.yahoo.com
> > _______________________________________________
> > Bioperl-l mailing list
> > Bioperl-l at lists.open-bio.org
> >
> http://lists.open-bio.org/mailman/listinfo/bioperl-l
> 
> --
> Jason Stajich
> Duke University
> http://www.duke.edu/~jes12
> 
> 
> 


__________________________________________________
Do You Yahoo!?
Tired of spam?  Yahoo! Mail has the best spam protection around 
http://mail.yahoo.com